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Biod 171 module 4 exam
Biod 171 module 4 exam
Course
Pharmacology (NUR 3145)
80 Documents
Students shared 80 documents in this course
University
Santa Fe College
Academic year: 2022/2023
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Learning/Module 4 Exam Questions
and Answers 2022/
Question 1
2 / 2 pts
True or False: Growth media is best suited for distinguishing between two similar
species of bacteria.
True
False
Growth media is designed to simply support (and not restrict) microbial growth.
A researcher is asked to determine if a sample contains Neisseria meningitides.
Knowing Neisseria meningitides is slow growing and other foreign microbes may also
be present in the culture, which type of media would be best suited?
Growth media
New BIOD 171 Portage
Learning/Module 4 Exam Questions
Question 2
2 / 2 pts
Learning/Module 4 Exam Questions
and Answers 2022/
Correct!
Selective media
Selective media. If additional foreign microbes may be present in your sample and
you know Neisseria meningitides is slow growing, you must restrict the unwanted
microbes while supporting the growth of Neisseria meningitides. Thus, you are
selectively encouraging one microbes growth, while selectively restricting others.
Selective and Differential media
Differential media
What are the requirements of a fastidious microbe?
A fastidious microbe is an organism with complex growth requirements
such that if absent it will not grow. Enriched medias thus contain these
specific and essential nutrients required for the growth of a particular
subset of microorganisms.
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Learning/Module 4 Exam Questions
Question 3
10 / 10 pts
Learning/Module 4 Exam Questions
and Answers 2022/
Seaweed extract
Seaweed (algae) extract.
Blood agar is which type of medium? Select all that apply.
Correct!
Differential
Blood agar can be used to differential between species based on its hemolytic activity.
Selective
Correct!
Enriched
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Learning/Module 4 Exam Questions
Question 6
2 / 2 pts
Learning/Module 4 Exam Questions
and Answers 2022/
Selective and Differential
Match the following hemolytic class with its description of activity.
1. Alpha hemolysis A. No change.
2. Beta hemolysis B. Greenish-brown color
3. Gamma hemolysis C. Distinct zone of clearing
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Learning/Module 4 Exam Questions
Question 7
5 / 5 pts
Learning/Module 4 Exam Questions
and Answers 2022/
Gram-Negative
Mycobacteria
Gram-Positive and Gram-Negative
Question 9
2 / 2 pts
True or False: Chocolate (cocoa) is not a component of Chocolate agar plates.
Correct!
True
The name is derived simply based on the color that actually comes from the
presence of ‘cooked’ (lysed) red blood cells in the media.
False
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Learning/Module 4 Exam Questions
Learning/Module 4 Exam Questions
and Answers 2022/
An unknown microbe is streaked onto a MacConkey agar plate. After an overnight
incubation at 37°C, growth is observed. Would a Gram stain be necessary? Why or why
not?
Your Answer:
No, a gram stain would not be necessary because only gram negative growth would be
observed on MacConkey agar plates.
No. A Gram stain would not be necessary, as only Gram-
Negative microbes will grow on MacConkey agar.
In an attempt to detect the presence of the pathogenic strain of E. coli O157:H7, a
researcher spread a culture onto a MacConkey agar with failed results. What type of
agar should they (correctly) try next? Why?
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Learning/Module 4 Exam Questions
Question 10
5 / 5 pts
Question 11
7 / 10 pts
Learning/Module 4 Exam Questions
and Answers 2022/
Mannitol salt agar will turn what color in the presence of the pathogenic strain
Staphylococcus aureus?
Correct Answer
Yellow
Pathogenic Staph aureus will turn the agar from red to yellow.
What is the process of spreading a bacterial culture onto a petri dish called?
Plating
Question 15
10 / 10 pts
Describe the primary advantage of using a petri dish over growing a liquid culture?
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Learning/Module 4 Exam Questions
Question 14
3 / 3 pts
Learning/Module 4 Exam Questions
and Answers 2022/
Your Answer:
When using a petri dish you can observe the growth of a sample in a specified region.
The primary advantage is that cells are held into place. When grown in a
nutrient broth, bacterial cells can multiply but are free to move around in
solution. When grown on agar within a petri dish the fixed in such as way
as to form colonies.
True or False: The visualization of colonies on a petri dish represents bacterial cells that
have often multiplied a million times over.
Correct!
True
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Learning/Module 4 Exam Questions
Question 16
2 / 2 pts
Learning/Module 4 Exam Questions
and Answers 2022/
To be considered a pure culture, the sample (1) can be traced back to a single cell
and (2)?
New BIOD 171 Portage
Learning/Module 4 Exam Questions
Learning/Module 4 Exam Questions
and Answers 2022/
The culture must also be free from external contaminants. Simply put, a pure
sample would never contain multiple bacterial species (ie) Strep and Staph.
When performing a quadrant streak, the sample is spread across the plate in such as
way as to form what?
A dilution gradient is formed. The resulting gradient should always contain
within it the growth of individual colonies.
In what phase of a dilution streak would you expect to find the highest concentration of
bacteria, P2 or P4?
New BIOD 171 Portage
Learning/Module 4 Exam Questions
Question 19
4 / 5 pts
Question 20
5 / 5 pts
Learning/Module 4 Exam Questions
and Answers 2022/
True or False. When performing a dilution streak a new (or sterilized) loop must be
used for each phase.
Correct!
True
Failure to do so would prevent the establishment of a dilution gradient, as the same
bacterial concentration would be spread across both phase regions.
False
The number of phases (3 vs. 4) and/or the number of times a loop passes through a
Either deviation is acceptable in practice provided the resulting gradient
contains within it the growth of individual colonies—if not, the
experiment must be repeated.
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Learning/Module 4 Exam Questions
Question 22
5 / 5 pts
Learning/Module 4 Exam Questions
and Answers 2022/
New BIOD 171 Portage
Learning/Module 4 Exam Questions
Question 23
2 / 2 pts
Learning/Module 4 Exam Questions
and Answers 2022/
4. Blood agar
Correct Answer: Blood agar. All other options (A, B and C) are all forms
of selective media, meaning they may potentially inhibit the growth of the
unknown sample. Although blood agar is considered a differential media,
New BIOD 171 Portage
Learning/Module 4 Exam Questions
Learning/Module 4 Exam Questions
and Answers 2022/
it is, most importantly, a non-selective media. Given the alternatives,
this is the best option.
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Learning/Module 4 Exam Questions
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Biod 171 module 4 exam
Course: Pharmacology (NUR 3145)
80 Documents
Students shared 80 documents in this course
University: Santa Fe College
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New BIOD 171 Portage
Learning/Module 4 Exam Questions
and Answers 2022/2023
Question 1
2 / 2 pts
True or False: Growth media is best suited for distinguishing between two similar
species of bacteria.
True
False
Growth media is designed to simply support (and not restrict) microbial growth.
A researcher is asked to determine if a sample contains Neisseria meningitides.
Knowing Neisseria meningitides is slow growing and other foreign microbes may also
be present in the culture, which type of media would be best suited?
Growth media
New BIOD 171 Portage
Learning/Module 4 Exam Questions
and Answers 2022/2023
Question 2
2 / 2 pts
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