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Catalase lab - intro purpose proced disc1

Biology enzyme worksheet
Course

Studies In Biology: Research (BIOL 493)

3 Documents
Students shared 3 documents in this course
Academic year: 2024/2025
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Keirra Battle
Tennessee College of Applied Technology Knoxville
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Biology 12 Name: _______________________

Enzymes Lab Partner:___________________

Catalase and Hydrogen Peroxide Lab Date of Lab:___________________

Block: ____

Introduction

Hydrogen peroxide (H 2 O 2 ) is a natural byproduct of the break down (and oxidation) of certain molecules, such

as fatty acids. Unfortunately, hydrogen peroxide is also toxic: it can destroy cell membranes. Hydrogen

peroxide is so effective at destroying membrane your white blood cells will actually produce it to fight bacteria!

In order to protect your cells, most reactions that produce hydrogen peroxide occur in a cellular membrane-

bound organelle called a peroxisome. (See page 50 and 55 in Mader.) If left on its own, hydrogen peroxide will

slowly decompose naturally into two harmless products, water and oxygen.

2 H 2 O 2 → 2 H 2 O + O 2

Peroxisomes contain large amounts of the enzyme catalase, a protein that increases the rate of hydrogen

peroxide decomposition. Catalase ensures that hydrogen peroxide is efficiently converted into water and

oxygen, leaving your cells unharmed. The cells in your liver cotain many peroxisomes in order to effectively

contain and decompose the large amounts of hydrogen peroxide produced from the breakdown of alcohol and

other substances. Obviously, catalase is an important protein made by your cells.

The optimal temperature of catalase is 37 0 C (body temperature) and the optimal pH is 7 (neutral). Prior to this

lab, pieces of liver (which contain catalase) have been subjected to: freezing, freezing then thawing, boiling,

6M Hydrochloric Acid, and 6M Sodium Hydroxide. You will observe how these factors affect enzyme activity

by adding these enzymes to hydrogen peroxide in a controlled experiment, and then observing how each

factor affects the rate of oxygen bubble production.

Purpose

In this laboratory investigation, you will:

  • Investigate and quantify the enzymatic reaction rate of hydrogen peroxide (H 2 O 2 ) decomposition.
  • Observe how temperature, pH, amount of enzyme, and reusing of enzyme affect enzyme activity.

Materials

 1 test tube rack

 8 test tubes

 1 thermometer

 1 timer/stopwatch

 1 test tube marker

 1 - 50mL beaker

 10mL hydrogen peroxide

 1 piece liver

 1 piece liver that has been pureed

 1 piece liver that has been frozen and then thawed

 1 piece liver that has been boiled

 1 piece liver that has been exposed to 6M hydrochloric acid (HCl)

 1 piece liver that has been exposed to 6M sodium hydroxide (NaOH, a base)

 HAZARD: hydrogen peroxide.

Contact with the eyes can cause serious long-term damage.

The solution is corrosive and can cause skin burns.

Slow decomposition of the solution in storage may lead to a build-up of pressure in sealed containers.

Eye contact: Immediately flush the eye with plenty of water. Continue for at least ten minutes.

Skin contact: Wash off with plenty of water.

Procedure

Prior to entering the lab:

 Create a procedural flow chart to use as a reference during the lab.

 Review how enzymes function and what role they play in reactions – review how amount of enzyme,

temperature and pH affect enzymes, and as a result, how they affect reaction rate. For each test tube

(there are 9), write a hypothesis describing what you believe will occur in each test tube and the reason

why you believe this will occur. Refer to your understanding of enzymes when explaining your

hypothesis. Hypotheses need to be in full sentences and act as answers to the question: How

will the enzyme-catalysed reaction be affected? For example: If there is no enzyme present, there

will be no observed reaction. Although the hydrogen peroxide will be decomposing, the reaction will

happen too slowly for any gas bubbles to be observed. Record your hypotheses and reasons in the

table provided.

 Predict the change in temperature that will be observed in each test tube: no change, increase,

decrease. Predict the rate of enzyme reaction when compared to test tube 2: no change, increase,

decrease. Record predictions in the data table.

During Lab:

1. Put on your safety goggles and apron.

2. Collect your test tube rack, 8 test tubes, and approximately 12mL of hydrogen peroxide in the 50ml

beaker. ***Note the hydrogen peroxide is currently decomposing into water and oxygen, just very

slowly!***

3. Label test tubes 1-8, which each number corresponding to the number on your data table.

4. Mark a line 1 cm (= 1) from the bottom on test tube 1 and fill with hydrogen peroxide from the

beaker. Record the volume. Test tube 1 will act as one control. Insert the thermometer into the

hydrogen peroxide of test tube 1 and record initial temperature. Remove thermometer.

5. After 3 minutes (You can start on step 6 and 7 while you wait.), insert the thermometer back into the

hydrogen peroxide of test tube 1 and record the final temperature. (Remove thermometer). Leave

test tube 1 until the end of the experiment.

6. Mark a line 1 cm from the bottom and a line 2 cm above that. Do this for all the test tubes. This will be

the “finish line” for the bubbles of gas produced.

7. Fill the test tube up to the first line with hydrogen peroxide.

8. Insert the thermometer into the hydrogen peroxide of test tube 2 and record the initial temperature.

Remove thermometer.

9. Obtain a piece of regular liver. Place it into test tube 2, while starting the timer.

10. Time how long it takes for the bubbles of gas to reach either the finish line. Record the time. *** If the

bubbles take more than 4 minutes, stop timing and record “> 4 min.”

11. Insert the thermometer into the hydrogen peroxide of test tube 2 and record the final temperature.

12. Repeat steps 6-11 with the other pieces of liver. Note that test tube 3 uses the liver after it is used in

the reaction of test tube 2. It is reused liver!

13. Clean-up.

Biology 12 – Lab pre and post assessment Name: _________________________

Catalase lab (on handout)

Prelab

 Procedure: Flowchart

- Excellent organization

- Diagrams clear and catch the eye

- Clear concise word commands for each step

- All steps included with creativity and originality

 Predication

- In table format

- For each test tube– make a hypothesis for what will

happen in each test tube, also include the reason for

your hypothesis

During

 Fully participates and follows the steps of the lab

including clean up

 Data table filled out – fully and neatly

 Experimental progress – detailed notes taken

 Answers the mystery question easily

Post lab

 Answer the discussion questions #1-2 correctly in full,

thoughtful sentences

 Describe what happened in each test tube – using

appropriate vocab and comparing to your hypotheses

 Conclusion – a paragraph that contains all of the

criteria written below

Beginning Developing Accomplished Exemplary

Prediction - not included - only some

predictions/reasons are

completed or the

predictions/reasons are vague

or unclear

- a simple hypothesis and/or

reason is made for each test

tube

- includes a table in which a

clear and thorough hypothesis

and reason is given for each

test tube

Flowchart –

due on the

day of the

lab

- Unorganized

- No diagrams

- Procedure is mostly

copied/not in own words

- Many steps are missing or

unclear

- Unorganized

- Diagrams are unclear

- Words commands are

unclear

- Some minor steps are

missing or unclear

- Organized

- Diagrams clear

- Clear word commands, but

not concise

- All steps included

- Excellent organization, easy

to follow.

- Diagrams clear and catch the

eye

- Clear, concise word

commands

- All steps included with

originality /creativity

Performance

and

Observations

(During the

lab)

-Just watched the lab be

performed and/or didn’t clean-

up

-Only ½ of the data is included

or almost impossible to read

data

-No log of experimental

progress

-Could not answer the

mystery question fully

-Needed some help to follow

the procedure and/or clean up

-Most data/observations

included or date recording

messy

-A couple of events are noted

in the Experimental progress

-Answered the mystery

question correctly when given

a bit of help

-Followed the procedure an

cleaned up

-All data/observations included

and neatly recorded

-Most important notes are

noted in the Experimental

Progress

-Answer the mystery question

correctly

-Followed the procedure

carefully including cleaning up

-Detailed/thorough

data/observations included

and neatly recorded

-Detailed log of experimental

progress.

-Answered the mystery

question easily and thoroughly

Summary

Questions –

(completed

after the lab)

--Fragments or point form

-Does not demonstrate a

basic understanding of

concept. Substantial errors

throughout

-Sentence structure lacking or

confusing

-Demonstrates a basic

understanding of concepts.

Errors and inconsistency

reveal some missing

understanding of the

concepts.

-Full sentences, well

structured

-Demonstrates a solid

understanding of concepts.

Most answers are correct with

only a few errors.

-Full sentences, well

structured

-Demonstrates complete and

in depth understanding of

concepts.

Conclusion

(completed

after the lab)

- Point form

- only 1 or 2 pieces of criteria

are covered but not thorough

- Personal opinions are

included “I like this lab” or,

“This lab was FUN!”

- Sentence structure lacking

- 3 or 4 pieces of criteria are

missing or incomplete

- Full Sentences, well

structured

- 1 or 2 pieces of criteria are

missing or incomplete

- Full Sentences, well-

structured and

thorough/detailed

All criteria listed below must

be included:

- Answers purpose - summary

of results and what they mean

- Interesting Findings

- States sources of error

- Connects results to big

picture (relevance)

- Asks new questions

-Suggests improvements or

changes to this lab procedure

Catalase

Lab

Biology 12 – Lab pre and post assessment Name: _________________________

Catalase lab (on handout)

Prelab

 Procedure: Flowchart

- Excellent organization

- Diagrams clear and catch the eye

- Clear concise word commands for each step

- All steps included with creativity and originality

 Predication

- In table format

- For each test tube– make a hypothesis for what will

happen in each test tube, also include the reason for

your hypothesis

During

 Fully participates and follows the steps of the lab

including clean up

 Data table filled out – fully and neatly

 Experimental progress – detailed notes taken

 Answers the mystery question easily

Post lab

 Answer the discussion questions #1-2 correctly in full,

thoughtful sentences

 Describe what happened in each test tube – using

appropriate vocab and comparing to your hypotheses

 Conclusion – a paragraph that contains all of the

criteria written below

Beginning Developing Accomplished Exemplary

Prediction - not included - only some

predictions/reasons are

completed or the

predictions/reasons are vague

or unclear

- a simple hypothesis and/or

reason is made for each test

tube

- includes a table in which a

clear and thorough hypothesis

and reason is given for each

test tube

Flowchart –

due on the

day of the

lab

- Unorganized

- No diagrams

- Procedure is mostly

copied/not in own words

- Many steps are missing or

unclear

- Unorganized

- Diagrams are unclear

- Words commands are

unclear

- Some minor steps are

missing or unclear

- Organized

- Diagrams clear

- Clear word commands, but

not concise

- All steps included

- Excellent organization, easy

to follow.

- Diagrams clear and catch the

eye

- Clear, concise word

commands

- All steps included with

originality /creativity

Performance

and

Observations

(During the

lab)

-Just watched the lab be

performed and/or didn’t clean-

up

-Only ½ of the data is included

or almost impossible to read

data

-No log of experimental

progress

-Could not answer the

mystery question fully

-Needed some help to follow

the procedure and/or clean up

-Most data/observations

included or date recording

messy

-A couple of events are noted

in the Experimental progress

-Answered the mystery

question correctly when given

a bit of help

-Followed the procedure an

cleaned up

-All data/observations included

and neatly recorded

-Most important notes are

noted in the Experimental

Progress

-Answer the mystery question

correctly

-Followed the procedure

carefully including cleaning up

-Detailed/thorough

data/observations included

and neatly recorded

-Detailed log of experimental

progress.

-Answered the mystery

question easily and thoroughly

Summary

Questions –

(completed

after the lab)

--Fragments or point form

-Does not demonstrate a

basic understanding of

concept. Substantial errors

throughout

-Sentence structure lacking or

confusing

-Demonstrates a basic

understanding of concepts.

Errors and inconsistency

reveal some missing

understanding of the

concepts.

-Full sentences, well

structured

-Demonstrates a solid

understanding of concepts.

Most answers are correct with

only a few errors.

-Full sentences, well

structured

-Demonstrates complete and

in depth understanding of

concepts.

Conclusion

(completed

after the lab)

- Point form

- only 1 or 2 pieces of criteria

are covered but not thorough

- Personal opinions are

included “I like this lab” or,

“This lab was FUN!”

- Sentence structure lacking

- 3 or 4 pieces of criteria are

missing or incomplete

- Full Sentences, well

structured

- 1 or 2 pieces of criteria are

missing or incomplete

- Full Sentences, well-

structured and

thorough/detailed

All criteria listed below must

be included:

- Answers purpose - summary

of results and what they mean

- Interesting Findings

- States sources of error

- Connects results to big

picture (relevance)

- Asks new questions

-Suggests improvements or

changes to this lab procedure

Catalase

Lab

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Catalase lab - intro purpose proced disc1

Course: Studies In Biology: Research (BIOL 493)

3 Documents
Students shared 3 documents in this course

University: Western Carolina University

Was this document helpful?
Biology 12 Name: _______________________
Enzymes Lab Partner:___________________
Catalase and Hydrogen Peroxide Lab Date of Lab:___________________
Block: ____
Introduction
Hydrogen peroxide (H2O2) is a natural byproduct of the break down (and oxidation) of certain molecules, such
as fatty acids. Unfortunately, hydrogen peroxide is also toxic: it can destroy cell membranes. Hydrogen
peroxide is so effective at destroying membrane your white blood cells will actually produce it to fight bacteria!
In order to protect your cells, most reactions that produce hydrogen peroxide occur in a cellular membrane-
bound organelle called a peroxisome. (See page 50 and 55 in Mader.) If left on its own, hydrogen peroxide will
slowly decompose naturally into two harmless products, water and oxygen.
2 H2O2→ 2 H2O + O2
Peroxisomes contain large amounts of the enzyme catalase, a protein that increases the rate of hydrogen
peroxide decomposition. Catalase ensures that hydrogen peroxide is efficiently converted into water and
oxygen, leaving your cells unharmed. The cells in your liver cotain many peroxisomes in order to effectively
contain and decompose the large amounts of hydrogen peroxide produced from the breakdown of alcohol and
other substances. Obviously, catalase is an important protein made by your cells.
The optimal temperature of catalase is 370C (body temperature) and the optimal pH is 7 (neutral). Prior to this
lab, pieces of liver (which contain catalase) have been subjected to: freezing, freezing then thawing, boiling,
6M Hydrochloric Acid, and 6M Sodium Hydroxide. You will observe how these factors affect enzyme activity
by adding these enzymes to hydrogen peroxide in a controlled experiment, and then observing how each
factor affects the rate of oxygen bubble production.
Purpose
In this laboratory investigation, you will:
- Investigate and quantify the enzymatic reaction rate of hydrogen peroxide (H2O2) decomposition.
- Observe how temperature, pH, amount of enzyme, and reusing of enzyme affect enzyme activity.
Materials
1 test tube rack
8 test tubes
1 thermometer
1 timer/stopwatch
1 test tube marker
1 - 50mL beaker
10mL hydrogen peroxide
1 piece liver
1 piece liver that has been pureed
1 piece liver that has been frozen and then thawed
1 piece liver that has been boiled
1 piece liver that has been exposed to 6M hydrochloric acid (HCl)
1 piece liver that has been exposed to 6M sodium hydroxide (NaOH, a base)

Students also viewed

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