Cheat Sheet enzyme lab

“Cheat Sheet” Enzyme Lab

Title: You can name the lab from the lab worksheet “Catalase Enzyme Lab” OR you can make

up your own name BUT, if made up, the name must tell the reader what the lab is about, so…

Chicken Liver Catalase and Hydrogen Peroxide Lab OR Liver Enzyme and Hydrogen Peroxide

OR Catalase and Hydrogen Peroxide OR….

Purpose: Read the background information and develop one or two sentences about the purpose

of this lab: The purpose of this lab was to ….”study the reaction of catalase from ______ on the

substrate _________ and to measure the relative influence of varying environmental factors such

as _____ and _______.” OR “observe the reaction of the enzyme catalase from chicken liver on

the substrate hydrogen peroxide, varying the pH and temperature…”

Procedure: Summarize the procedure in a few sentences. OR you can completely copy the

procedure from the lab BUT if the lab was changed from the procedure, MAKE SURE you

include the changes in your report.

Observations: Include a Data Table (can be exactly like one from lab, BUT NEATER)

ALSO need to include 2 GRAPHs. (Similar to graph on back of Enzyme Review worksheet)

Graph 1: Relative Rate of Chicken Liver Catalase on Hydrogen Peroxide while varying

Temperature

Graph 2: Relative Rate of Chicken Liver Catalase on Hydrogen Peroxide while varying pH.

Remember: Every graph needs: Title (see above), Both Axes need to be labeled (see below),

appropriate scale/numbering and MUST include UNITS.

For both Graphs: Dependent Variable (thing you measured) (Y axis) is “Relative Rate of

Enzyme Action” You will put down reaction rate from your data table. Remember, Part A was

arbitrarily set to “4” . All others are based on that, so Range will be 0 to 10.

Independent Variables:

For Temperature Graph, X axis is Temperature in oC . You have ice bath data from 0oC (or

5 or 10 or whatever you measured); You have “Body Temperature” data (about 40oC) and you

have “Boiled Liver data” ( 100 oC). ALSO: Include data from Part A, assuming “Normal

Liver” was done at room temperature which is 25 oC. Use a legend to show the different data.

For pH graph: X axis is pH (Range of data is 1 to 14. USE THE DATA FROM MS.

RANNEY’S DEMO for the 3 different conditions DEMO BY MS. RANNEY and put that data

in your data table AND on the graph.

Discussion/Analysis: In this section, at a minimum you must write a sentence describing your

results AND the answer to EVERY QUESTION asked on the lab worksheet. You can add

additional sentences to explain what was going on and how it relates to your understanding of

enzymes. You should use your textbook, the enzyme review worksheet, and the internet to make

sure you have discussed EVERY item. If you did not get expected results, you must state so and

indicate what you think happened that made you get unexpected or wrong results. (This is your

Studies In Biology: Research (BIOL 493)