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SBTB031 2022 Study guide

Industrial biotechnology study guide

Course

industrial biotechnology (sbtb031)

32 Documents

Students shared 32 documents in this course

University

University of Limpopo

Academic year: 2021/2022

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Kamogelo disegoane

University of Limpopo

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STUDY GUIDE 2022

DEPARTMENT OF BIOCHEMISTRY, MICROBIOLOGY AND BIOTECHNOLOGY

SBTB

Industrial Biotechnology

Number of credits: 32

Semester offered: 1

Prepared by: Dr NN Phasha University of Limpopo

Facilitators: Dr NN Phasha University of Limpopo Ms M Mogashoa Mrs MR Lekganyane Mr L Ramoba

TABLE OF CONTENT

1 MODULE INFORMATION...............................................................................................
- 1 Overview of the module..............................................................................................
  - 1.1 Purpose and learning objectives..............................................................................
- 1 Learning outcomes......................................................................................................
- 1 Entry assumptions.......................................................................................................
- 1 Structure......................................................................................................................
- 1 Presentation methodology...........................................................................................
- 1 Assessment criteria.....................................................................................................
- 1 Information on Assessment Questions........................................................................
- 1 Prescribed book and suggested reading......................................................................
2 FACILITATION SCHEDULE............................................................................................
- 2 Facilitators...................................................................................................................
- 2 Content for theoretical work.......................................................................................
- 2 Assessment dates for theoretical work........................................................................
- 2 Schedule content for practical work...........................................................................
- 2 Assessment dates for practical work...........................................................................
3 DETAILED CONTENT.....................................................................................................

1 Assessment method All assessments will count towards the final mark.

Theory mark: contribute 40% towards the final mark and consists of

Two tests (35%) and class quiz and assignments (5%)

Practical mark: contribute 20% towards the final mark and consists of a practical report (05%) and practical test (15%)

Examination mark: contribute 40% towards the final mark and consists of an online exam.

Students will be required to obtain at least 40% for the semester mark in order to qualify to write the examination.

Irrespective of the final mark achieved, students have to obtain at least 40% in the summative assessment (examination paper) to pass the module.

Students will be required to write a supplementary examination if:  they obtained a final mark of 50% or more but obtained less than 40% in the summative assessment (examination); this is called subminimum.  they obtained a final mark of between 45% and 49% (both inclusive).

A final mark of 50% or higher is considered a pass and 75% and higher a pass with distinction.

1 Information on Assessment Questions Based on the school assessment policy, outcomes of the third year module are based on 40% knowledge, comprehension and application questions whereas 60% is on analysis, evaluation and synthesis type questions.

Guidelines on answering questions:  Why - is for questions that require reflection and understanding of the basic concepts.  Describe and How - are mostly used when the student is required to write out what/how a particular process is/occurs. The How question can be answered by listing of the steps in that process. The steps should be logical and chronological.  Discuss: mostly tests an understanding of the concepts. The students must show ability to use own words to report on a particular concept. The answer must be characterized by statements that build on each other and a flow of ideas or facts should be evident.

1 Entry assumptions

When entering this module, students are expected to have a good knowledge of (Introduction to Biotechnology – (SBTA022) or Fundamental Microbiology –

(SMIA021). These sections will not be discussed in detail during the lectures.

1 Structure

Duration of module: 15 weeks of theoretical and practical classes (24 January – 13

May 2022) Mid-year summative examinations start on the 23 May 2022

Lectures per week: 2 (90 minutes/ lecture), presented on Monday Blackboard 11:10-

12:

Thursday Blackboard 07:30- 09:10)

Practicals per week:1, Blackboard (Thursday at 14h00)

1 Presentation methodology

This module is presented through pre-recorded study material and

discussions/information sessions on blackboard collaborate ultra. One on one

student consultations will also be conducted through blackboard collaborate on an

appointment basis.

It is incumbent on the student to engage study material posted for each class and

attend discussion/ information session through blackboard collaborate ultra.

Practical classes will be conducted to aid your understanding of the theory work.

Detailed videos of experiments will be posted on blackboard and the students are

expected to watch the videos, engage with the instructor and write submit practical reports.

1 Recommended books

2 Basic Biotechnology 2nd Edition by Colin Ratledge (Editor), Bjorn Kristiansen.

3 Industrial Microbiology, Michael J Waites, Neil L. Morgan, John S Rockey, and Gary

Higton

4 BROCK: Biology of microorganisms, 15th edition by Madigan MT, Bender KS, Buckley

DH, Sattley WM and Stahl DA, 2019.

5 Biotechnology: An introduction, 2nd edition by SR Barnum, 2005.

6 Principles of Microbiology, 2nd edition by R Atlas, 1996

7 Microbiology An introduction, 5th edition by Tortora GJ, Funke BR and Case CL,

8 SBTB031 class notes

9 Schedule content for practical work

Week 1

Week 2

Week 3

Week 4

Week 5-

Practical 1: Orientation and familiarization with rules, equipment and other objects Practical 2: Substrate induced production of an industrial enzyme-preparation of inoculum Practical 3: Substrate induced production of an industrial enzyme-submerged Practical 4: Substrate induced production of an industrial enzyme-lipase assay Practical 5: Yogurt and wine production Practical 6: Yogurt and wine production (continuation) Practical 7: Yogurt and wine production (continuation)

9 Assessment dates for practical work Assessment occurs through practical reports and test. Reports to be submitted

approximately 7 days after a practical class was conducted. The test will be written

on a pre-determined date.

10 DETAILED CONTENT

CONTENT FACILITATOR

PART I

Definition and history of biotechnology Range of definitions and their implications Historical use and knowledge of biotechnology

NN

Overview of the products of biotechnology: Green Biotechnology, White Biotechnology, Red Biotechnology, etc. NN
Microbial growth kinetics NN
Enzyme technology Sources of enzymes Enzyme production Enzyme immobilisation

NN

Bioreactors and bioreactor designs NN
Biocatalysis and Biotransformation NN
Downstream processing of industrial product and biological remediation of industrial waste Product purification Recovery Effluent treatment Disposal

NN

PART II

Putting Industrial microorganisms to work substances produced by microbial cells isolation of microorganisms strain improvement

M

Major products for the health industry antibiotics vitamins amino acids microbial enzymes

M

Major products for the food and beverage industry alcoholic fermentation dairy products single cell protein

M

The techniques of genetic engineering principle of genetic engineering diverse hosts cloning vectors

M

Practical applications of genetic engineering production of proteins vaccines genetic engineered products

M

Food preservation and microbial growth microbial growth food spoilage and preservation

M

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