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Preparation of buffers and determination of p H using p H metre
Bsc botony (Botony)
University of Calicut
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Expt. No......
HERO
AJM:
bH mete
B
PRLPARATION 0F
BUFFCRS AND DEIKMING
pH UStNG pHMGTGK
he oufheI solaton ia the one which zesst
change
Sn 4 when Qcid cx
bae added to it cuualy
made cuh weak
aid ancla caniugate bae Guler hlays an Jmetant
1olin inslvo
Lako. Bataue 'ej the
biotgical nndion cant cherate
eitientty
land ebtimally ot a ulde qarge a bH. pry
enstant þh it maintained
Page No....
arnd delorine bh cuing the
Date .J3:18....
The esistance g bufer to chanqe n ph on addition
Gmall ameunt e alkali sr aid ic knewn ay bller achn
H
þH neter a peternlcndes hat measunes
hyden ion ancentratian and i delioed ar the begaue
logasthrm e hydasgen ion tondentnatón.
diftentes o þeternial betunen the glais dachade. and
Dlorente glectode istmaasuned , amjali{ed ard Carsented into
en meter p4 e the kalution Can be determinal
Fglas Re
0:059)
at se
Teacher's Signature...
'n cell ad body id by the
yaious kioogical subtlanaes [uch.
N phesphate, bianbonate and catbenate ptc.
Expt. No.... bH METER Page No... platinum wire
inlei for kcl
led sotution
- internal electrode
quautity But4ele acid qm h00l Citrate busder bus4t acetate A buyger citric B aoid. Socium plosphale day dr ogerphosphau di basic Phosphole MOwo-base A1839rm Aceticawd quauhhy -55 uÀn alkali a e ocium citale Socium hydrogen 63-65qm in loo m on aceto PH 1 4
Expt. No.. HERO
PRoeDURE:
Collectod
RESULIS
####### tnf buhers upe made and kebt n
cnde Date Page No.... Then had brn
checked by wsing ampla.
####### samblk1 k eoladaink, qanden sofl,
Butlen milk Drinking DISCUSSLOAL hñ bugex ucre trchared with cahective acid. and baes. Acd and alkaltne wre Wed to ad<ust the t and the value e hH were takulated and also the Ho toml 6ambk kelutce, butes mik ate luas checkeod utnq. An atid delined as a subslance that yldds H lon bage alkali ferms 9H long. Acide ame roen donans and baues ane babton acttbBos the strahgdn ey an atid bae i detoxninad by the de¡nee H Îphizalion agutous dolaton he weakegt acid has shongtt ccnËugak O mlxture is nethey acid gor base his hhenomlnpn wLak ad ond i bose. ated beutralization hu acchc er bate natne a Apluthon ib exhneced in degms oH in ton tentiatten lhe coloug fhe qoluien dehtnce on the pH SOme indieators are (oononly ued seh ar batmothymd blue phenolbthaltin and mtoyl nange. Th indietors ane in oluton cenit ea mixAure Teacher's Signature... bat. hysEngett aad bas the sgakust denjgte ie base, 4 weal bae and it salt.
Preparation of buffers and determination of p H using p H metre
Course: Bsc botony (Botony)
University: University of Calicut
