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TERM Paper
Biochemistry (MC 102)
Bukidnon State University
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Exploring the Metabolic and Probiotic Potential of Bifidobacteria Using a
Non-Target Metabolomics-Based Screening Technique
Necka Earl D. Amolo Department of Food Science, Central Mindanao University CHY 47: Biochemistry Ma’am Gina B. Barbosa February 19, 2023
Introduction
The word "probiotic" means "for life" in Greek. For life, means every element or organism that improves the host's state of health. Probiotic consumption is growing in popularity as a result of the general public's growing health consciousness as probiotics have a lot of beneficial effects, the rationale behind why consumers are drawn to probiotic-containing products. The global market for functional foods is presently dominated by probiotic products, accounting for over 65% of the industry, and is expected to reach a value of USD 64 billion this year (Reid et al., 2019). Bifidobacterium is one of the most widely utilized probiotic strains, along with the Lactobacillus genera (IPA Europe, 2022).
Bifidobacteria are commensal to the human gut, and numerous isolates and strains of bifidobacteria from the human gut have demonstrated health-promoting properties (Turroni et al., 2019). Bifidobacterial variety is abundant in nature, but significant in vitro and in vivo testing is necessary to determine whether a newly discovered bifidobacterial strain has probiotic qualities or is even acceptable for use as a probiotic strain in food and industrial processes (Das et al., 2022). Yet, it would be far more practical and economical to conduct in vitro tests to screen it first for possible candidates containing desirable features before doing several animals or even human trials to examine the in vivo function of probiotics, using a non-target metabolomics-based screening method Li et al. (2022).
Metabolomics
High-throughput omics technologies, including genomics, transcriptomics, proteomics, and metabolomics, are already proving to be essential tools for understanding an organism's biology and how it responds to environmental signals or genetic alterations. Metabolomics is one of these techniques that is primarily used to investigate metabolites, which are typically tiny molecules of less than 1000 Da, within cells, biofluids, tissues, or organisms (Troisi et al., 2020). Targeted and untargeted metabolomics are the two forms that are now available. Whereas focused metabolomics focuses on identifying and measuring the chemicals of interest, the untargeted analysis evaluates (ideally) all the metabolites in a given sample (Lelli et al., 2021). Liquid chromatography-mass spectrometry (LC-MS), which has the advantages of high throughput, mild ionization, and good coverage of metabolites, is frequently employed as the platform for metabolomics investigations (Van Der Laan et al., 2020).
References
Colli-Dula, R. C., & Ruiz-Hernández, I. M. (2022). Applications of Omics Approaches to
Decipher the Impact of Contaminants in Dolphins. IntechOpen EBooks. doi/10.5772/intechopen.
Das, T., Pradhan, S., Chakrabarti, S., Mondal, K. C., & Ghosh, K. (2022). Current status of
probiotics and related health benefits. Applied Food Research, 2 (2), 100185. doi/10.1016/j.afres.2022.
IPA Europe. (2022, May 12). What are probiotic microorganisms? – Ipa Europe. Ipa Europe.
ipaeurope/probiotics/what-are-probiotics/
Lelli, V., Belardo, A., & Timperio, A. (2021). From Targeted Quantification to Untargeted
Metabolomics. IntechOpen EBooks. doi/10.5772/intechopen.
Li, B., Liu, K., Kwok, L., Guo, S., Bai, L., Yang, X., & Chen, Y. (2022). Development of a non-
target metabolomics-based screening method for elucidating the metabolic and probiotic potential of bifidobacteria. Innovative Food Science and Emerging Technologies, 77 , 102971. doi/10.1016/j.ifset.2022.
Powell-Wiley, T. M., Poirier, P., Burke, L. E., Després, J., Gordon-Larsen, P., Lavie, C. J., Lear,
S. A., Ndumele, C. E., Neeland, I. J., Sanders, P., & St-Onge, M. (2021). Obesity and Cardiovascular Disease: A Scientific Statement From the American Heart Association. Circulation, 143 (21). doi/10.1161/cir.
Reid, G., Gadir, A. A., & Dhir, R. (2019). Probiotics: Reiterating What They Are and What They
Are Not. Frontiers in Microbiology, 10. doi/10.3389/fmicb.2019.
Troisi, J., Ciacci, C., Colucci, A., Pierri, L., Scala, G., Symes, S. J. K., Jones, C., & Richards, S.
M. (2020). Metabolomics in genetic testing. Elsevier EBooks, 85–153. doi/10.1016/bs.acc.2019.07.
Turroni, F., Duranti, S., Milani, C., Lugli, G. A., Van Sinderen, D., & Ventura, M. (2019).
Bifidobacterium bifidum: A Key Member of the Early Human Gut Microbiota. Microorganisms, 7 (11), 544. doi/10.3390/microorganisms
Van Der Laan, T. P., Dubbelman, A. C., Duisters, K., Kindt, A. S., Harms, A. C., & Hankemeier, T.
(2020). High-Throughput Fractionation Coupled to Mass Spectrometry for Improved Quantitation in Metabolomics. Analytical Chemistry, 92 (21), 14330–14338. doi/10.1021/acs.analchem.0c
TERM Paper
Course: Biochemistry (MC 102)
University: Bukidnon State University
