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Blood bank wall notes
internship notes
Course
Medical Technology (BSMT1)
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Emilio Aguinaldo College
Academic year: 2023/2024
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Blood Bank | Wall Notes (by: juwel)
Blood Component Volume Storage Expiration Date Quality Requirements
Whole Blood (WB) 450 – 500 mL +2 to +8°C Closed System:
CPDA-1: 35 days
CPD/ACD: 21 days
Hemoglobin (Hgb): Minimum 45g/unit
Hematocrit (Hct): 0 to 0.
pH: > 6.
Sterility: No growth
Modified Whole Blood 350 – 400 mL +2 to +8°C Same as WB
(Only for PRBC request)
Same as WB
Packed Red Blood Cells (PRBC) 250 – 300 mL +2 to +8°C Same as WB
Open System:
24 hours after packing
Same as WB
Platelet Concentrate (PC) 50 – 70 mL +20 to +24°C 3 – 5 days (in continuous
agitation)
Platelet count: at least 5 10
**Presence of platelet ribbon/platelet swirling
**Stand for an hour before agitation
Fresh Frozen Plasma (FFP) 200 – 250 mL - 30 to - 39°C 1 year
When thawed:
6 hours after thawing
Factor VIIIc level: > 70 IU/100 mL
*Dispose any lipemic or chylous samples
Cryoprecipitate 15 – 20 mL - 30 to - 39°C Same with FFP Factor VIIIc level: > 70 IU/100 mL
Fibrinogen: > 140 mg/unit
VWF: > 100 IU/unit
Blood Typing
Forward Grouping – uses known Anti-A, Anti-B, and Anti-D reagents to demonstrate the presence or absence of the A, B, and Rh antigens on the patient’s red blood cells.
Reverse Grouping – uses known A1 and B red blood cell reagents to demonstrate the corresponding presence or absence of anti-A and anti-B in the patient’s serum or plasma.
Forward ABO Blood Grouping Reverse ABO Blood Grouping Blood Type
Anti-A Anti-B Known A Known B
0 0 + + O
+ 0 0 + A
0 + + 0 B
+ + 0 0 AB
Crossmatching
- Compatibility testing wherein donor red cells are incubated with recipient’s serum.
- Used to demonstrate ABO incompatibility and clinically significant antibodies to red cell antigen.
(1) Immediate Spin
- 2 drops serum (patient) + 1 drop RCS (donor)
- Centrifuge for 30 – 40 secs
- Read and record
(2) Thermo Phase
- Add 2 drops of LISS
- Mix, then incubate for 10 – 15 mins @ 37°C
- Centrifuge for 30 – 40 secs
- Read and record
(3) AHG
- Wash 3x
- After 3rd (last) wash, blot tube in a gauze pad/tissue paper to absorb excess saline
- Add 2 drops of AHG
- Centrifuge for 30 – 40 secs
- Read immediately macroscopically and microscopically
- Record results
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Blood bank wall notes
Course: Medical Technology (BSMT1)
431 Documents
Students shared 431 documents in this course
University: Emilio Aguinaldo College
Was this document helpful?
Blood Bank | Wall Notes (by: juwel)
Blood Component
Volume
Storage
Expiration Date
Quality Requirements
Whole Blood (WB)
450 – 500 mL
+2 to +8°C
Closed System:
CPDA-1: 35 days
CPD/ACD: 21 days
Hemoglobin (Hgb): Minimum 45g/unit
Hematocrit (Hct): 0.35 to 0.45
pH: > 6.5
Sterility: No growth
Modified Whole Blood
350 – 400 mL
+2 to +8°C
Same as WB
(Only for PRBC request)
Same as WB
Packed Red Blood Cells (PRBC)
250 – 300 mL
+2 to +8°C
Same as WB
Open System:
24 hours after packing
Same as WB
Platelet Concentrate (PC)
50 – 70 mL
+20 to +24°C
3 – 5 days (in continuous
agitation)
Platelet count: at least 5.5x1010
**Presence of platelet ribbon/platelet swirling
**Stand for an hour before agitation
Fresh Frozen Plasma (FFP)
200 – 250 mL
- 30 to -39°C
1 year
When thawed:
6 hours after thawing
Factor VIIIc level: > 70 IU/100 mL
*Dispose any lipemic or chylous samples
Cryoprecipitate
15 – 20 mL
- 30 to -39°C
Same with FFP
Factor VIIIc level: > 70 IU/100 mL
Fibrinogen: > 140 mg/unit
VWF: > 100 IU/unit
Blood Typing
Forward Grouping – uses known Anti-A, Anti-B, and Anti-D reagents to demonstrate the presence or absence of the A, B, and Rh antigens on the patient’s red blood cells.
Reverse Grouping – uses known A1 and B red blood cell reagents to demonstrate the corresponding presence or absence of anti-A and anti-B in the patient’s serum or plasma.
Forward ABO Blood Grouping
Reverse ABO Blood Grouping
Blood Type
Anti-A
Anti-B
Known A
Known B
0
0
+
+
O
+
0
0
+
A
0
+
+
0
B
+
+
0
0
AB
