- Information
- AI Chat
Biochem B5 Summary of Test Lipids
BS Nursing (BSN)
Recommended for you
Preview text
TESTS Spotting Effect Solubility BROMINEWATER NINHYDRIN SODA LIME AMMONIUM MOLYBDATE BURCHARD TEST FOR Presence of lipids Solubility of different solvents Double bonds of lipids (fatty acid or triglyceride) Glycerol Glycolipids acids Lipid with amino acid Sphingolipids Phospholipids and phosphatidylserine Cholesterol SUMMARY OF TESTS REAGENTS Methylene chloride, Bromine water KHS04 solid conc., HzSOr. Ninhydrin reagent Soda HNOSand ammonium solution Acetic anhydride (onc., H2S04, methylene chloride PRINCIPLE High melting point Dissolves in organic solvents, not in H0 Addition reaction in carbon double bonds Dehydration Dehydration and condensation Oxidative deamination and condensation Deamination Hydrolysis and precipitation Dehydration and Oxidation RESULT Varies translucent spot when dry Varies miscibility Bromine layer becomes colorless Burned fat (grease) odor Purple interphase solution Red to blue litmus paper Yellow precipitate Emerald green solution