Skip to document

Buffers Exercises

exercises
Course

organic- bio chemistry (CHEM153L)

145 Documents
Students shared 145 documents in this course
Academic year: 2013/2014
Uploaded by:
Anonymous Student
This document has been uploaded by a student, just like you, who decided to remain anonymous.
Xavier University - Ateneo de Cagayan

Comments

Please sign in or register to post comments.

Preview text

Exercises

  1. How would you prepare 500 mL of 0 M phosphate buffer solution, pH 6 from 0 M solutions of acid and salt?

First, I would calculate the mass of the acid and its salt needed. Below is how I calculated it.

Next, I would weigh the calculated acid and salt then dissolve it in 250mL of distilled H2O in a beaker. Once both are fully dissolved, I would use a pH meter to obtain the pH of the solution. I would then adjust it with either a strong base or acid if I do not attain the pH level I desire. After, I would transfer the solution to a volumetric flask then dilute to mark. Lastly, I will transfer it to an Erlenmeyer flask and cover it with a stopper. 2. What will be resulting pH of the buffer in Question # 1 if the following are added: a. 1 mL of 1 M HCl

b. 1 mL of 1M NaOH

  1. Compare the results in question # 2 if these reagents were added to 500 mL of distilled water instead? Interpret your results.

The pH that would occur would be different. HCl and NaOH completely dissociate when added to water since they are both strong acids and bases. The conjugate base will react with the additional acid, and the acid will react with the added base, resulting in a pH shift in a buffer that is not very significant.

Was this document helpful?

Buffers Exercises

Course: organic- bio chemistry (CHEM153L)

145 Documents
Students shared 145 documents in this course
Was this document helpful?
Exercises
1. How would you prepare 500.0 mL of 0.050 M phosphate buffer solution, pH 6.7 from
0.20 M solutions of acid and salt?
First, I would calculate the mass of the acid and its salt needed. Below is how I
calculated it.
Next, I would weigh the calculated acid and salt then dissolve it in 250mL of
distilled H2O in a beaker. Once both are fully dissolved, I would use a pH meter
to obtain the pH of the solution. I would then adjust it with either a strong base or
acid if I do not attain the pH level I desire. After, I would transfer the solution to a
volumetric flask then dilute to mark. Lastly, I will transfer it to an Erlenmeyer
flask and cover it with a stopper.
2. What will be resulting pH of the buffer in Question # 1 if the following are added:
a. 1.0 mL of 1.0 M HCl